Peptide Testing & Quality Assurance
Every research peptide we supply undergoes multi layer analytical verification using advanced pharmaceutical grade instrumentation.
This ensures identity, purity, potency, and consistency not just on paper, but at the molecular level.
Our Testing Question
Is this peptide exactly what it claims to be, clean, accurate, and reproducible?
Our 10-Step Testing Protocol
UPLC / HPLC with PDA Detector
Purity, Identity & Consistency
Ultra Performance Liquid Chromatography (UPLC) with a Photodiode Array (PDA) detector is the foundation of peptide quality testing.
What it verifies
- ✓HPLC purity – Confirms the percentage of the target peptide
- ✓Assay (potency) – Ensures correct concentration
- ✓Related substances – Detects peptide fragments and synthesis by-products
- ✓Retention time identity – Confirms the peptide behaves exactly as expected
Why this matters
High purity and consistent retention time ensure the peptide is chemically correct and reproducible batch to batch.
LC–MS (Single Quadrupole, ESI)
Molecular Weight & Intact Mass Confirmation
Liquid Chromatography–Mass Spectrometry (LC–MS) confirms the exact molecular mass of the peptide.
What it verifies
- ✓Intact mass confirmation
- ✓Molecular weight accuracy
- ✓Impurity mass detection
Why this matters
Even a single missing or altered amino acid changes the molecular weight. This step confirms the peptide is structurally correct, not just "close enough."
LC–MS/MS (Triple Quadrupole)
Sequence Confirmation & Fragment Analysis
Triple quadrupole LC–MS/MS goes deeper, breaking the peptide into fragments and verifying its sequence.
What it verifies
- ✓Sequence confirmation (MS/MS)
- ✓Fragment identification
- ✓Cross-contamination & carryover detection
Why this matters
This ensures the amino-acid sequence is exact, and that no residual peptides from previous runs contaminate the batch.
Gas Chromatography with Headspace Sampler
Residual Solvent Safety
Peptide synthesis involves organic solvents. Gas Chromatography (GC) ensures they're gone.
What it verifies
- ✓Residual solvent levels
- ✓Compliance with accepted analytical thresholds
Why this matters
Confirms peptides are clean, safe, and free from synthesis solvents.
Ion Chromatography
Counterion & Inorganic Ion Verification
Ion Chromatography identifies the counterions paired with peptides.
What it verifies
- ✓Counterions such as TFA, acetate, or chloride
- ✓Inorganic ion profile
Why this matters
Counterions affect stability, solubility, and analytical consistency.
Karl Fischer Titration
Water Content Accuracy
This test measures precise moisture content in lyophilized peptides.
What it verifies
- ✓Residual water percentage
Why this matters
Too much moisture can degrade peptides. This ensures stability and shelf-life reliability.
Amino Acid Analyzer
Peptide Composition & Content
This confirms peptide composition after hydrolysis.
What it verifies
- ✓Amino acid composition
- ✓Peptide content determination
Why this matters
It provides independent confirmation that the peptide matches its declared structure.
ICP–MS
Heavy Metal & Elemental Impurity Testing
Inductively Coupled Plasma–Mass Spectrometry detects metals at parts-per-billion levels.
What it verifies
- ✓Heavy metals (lead, mercury, cadmium, arsenic)
- ✓Trace elemental impurities
Why this matters
Ensures peptides are clean at the elemental level, not just organic purity.
pH Testing
Chemical Stability Check
This test measures the pH level of the final peptide solution to ensure proper chemical environment for stability.
What it verifies
- ✓Final solution pH (when applicable)
Why this matters
pH affects stability, degradation rate, and compatibility in research environments.
Stability Chambers
Real-World & Accelerated Stability
Controlled chambers simulate time and temperature exposure.
What it verifies
- ✓Real-time stability
- ✓Accelerated stability under stress conditions
Why this matters
Confirms peptides maintain purity and potency over time, not just on release day.
Guaranteed Quality. Verified Results.
Every batch tested. Every molecule verified. Your confidence, our commitment.
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